Step
1.Blast Search
->
2.Select sequences
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3.SALAD interactive analysis
->
4.Results
Interactive SALAD analysis using multi-fasta generated by BLAST
BACK
Program:
blastp (Amino Acid query vs Amino Acid database)
blastx (DNA query vs Amino Acid database)
Query:
Search query by annotated protein information
Database:
Select species -specific search-
Uniprot(Swiss-Prot & TrEMBL) plant division -wide search-
Uniprot(Swiss-Prot & TrEMBL) plant division contains about 800,000 sequences
Select Rice Annotation data
Please select one of the following database for rice annotation.
RAP
IRGSP/RAP build 5 annotation data
MSU
Release 6.1
Deselect all
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Select all
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Collapse all
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Expand all
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Monocot
Oryza sativa (RAP-DB or MSU)
Brachypodium distachyon (JGI)
Sorghum bicolor (JGI)
Zea mays (Maize Genome Sequencing Project)
Eudicot
Arabidopsis thaliana (TAIR9)
Carica papaya (ASGPB)
Glycine max (JGI)
Medicago truncatula (MGSC)
Populus trichocarpa (JGI)
Vitis vinifera (IGGP)
Fragaria vesca (SGSC)
Lycophyte
Selaginella moellendorffii (JGI)
Moss
Physcomitrella patens (JGI)
Green alga
Chlamydomonas reinhardtii (JGI)
Ostreococcus tauri (JGI)
Volvox carteri (JGI)
Red alga
Cyanidioschyzon merolae (Genome Project)
Yeast
Saccharomyces cerevisiae (SGD)
Help page
Detailed options
Maximum number :
10
20
30(default)
50
Maximum number of sequences in each species to display
Expect threshold :
10
1
0.1
0.01(default)
1e-5
1e-10
1e-20
1e-50
E-value threshold
Filter :
On
Off
Mask off segments of the query sequence that have low compositional complexity
Word size :
Default value is 11 for blastn, and 3 for the other programs.
Mask :
Mask lower case letters
E-value points :
Count the number of hits at each E-value
Enter E-value points.(e.g., 1e-10,1e-20).
Multiple number should be separated with a comma(,).
Criterion for
caluclating MR :
Total score (order of priority:1st=Total score, 2nd=E-value)
Max score (order of priority:1st=Max score, 2nd=E-value)
E-value (order of priority:1st=E-value, 2nd=Total score)
Query coverage (order of priority:1st=Query coverage, 2nd=Total score)
Total Score: By the sum of scores from all HSPs from the same database sequence
Max Score: By the bit score of HSPs, similar to Expect Value
E-value: Expect Value
Query Coverage: By the percent of length coverge for the query